http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013534415-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-952 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15 |
| filingDate | 2011-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2013534415-A |
| titleOfInvention | N-terminal rule protease identification method and use thereof |
| abstract | The present invention provides a cell-based assay for detecting protease activity. In this assay, the cells are genetically engineered to express a protease substrate that preferably has at least one label at its C-terminus. Cleavage of the substrate by a protease that identifies it results in a C-terminal fragment and an N-terminal fragment, and the fragment with the label undergoes degradation by the ubiquitin proteasome. This assay measures label loss due to degradation of the fragment to which it is attached. A cell-free assay for detecting protease activity is also disclosed. In this cell-free assay, the protease substrate is expressed in a solution containing elements of the ubiquitin-proteasome pathway for degradation of the fragment. This assay measures the disappearance of the label attached to the fragment resulting from cleavage by the protease. [Selection] Figure 1 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019516379-A |
| priorityDate | 2010-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 235.