| abstract |
Embodiments of chemically defined cell culture media that do not contain any serum, contain nutrients and growth factors, and cells such as mesenchymal stem cells for culturing cells such as mesenchymal stem cells Methods of using cell culture medium embodiments to increase the population while maintaining a pluripotent phenotype, and mesenchymal stem cell chondrogenesis and by mixing differentiation factors into the cell culture medium embodiments A method of inducing bone formation. Another broad object of the present invention is a method that avoids the step of culturing cells such as MSCs on a negatively charged plastic surface such as a negatively charged polystyrene plastic surface and coating the surface with fibronectin or the like. possible. |