| abstract |
That at least one chromatography step is performed using a multimodal resin, the growth factor protein binds to the multimodal resin at a pH between 4 and 6.2, and the growth factor protein has a pH higher than 6.3. Elution, and elution of the growth factor protein is improved by the addition of arginine and / or sodium chloride to the elution buffer, following the multimodal resin step, a yeast-derived affinity ligand resin step is performed, A method for purifying a growth factor protein in a series of purifications using chromatography characterized in that a product with a purity higher than 90% results. |