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filingDate 2011-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2013-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2013523143-A
titleOfInvention Methods for isolating and purifying nucleic acids
abstract The present invention is a method for isolating and purifying nucleic acid, preferably comprising genomic DNA, from a biological sample, said method using a lysis buffer comprising a source of anionic surfactant ions. Lysing the sample, optionally disrupting RNA present in the lysate, precipitating the surfactant ions from the lysate, and sizing the nucleic acid from the precipitate and additional contaminants It relates to a method comprising the step of separating by exclusion chromatography. The invention further relates to a lysis buffer, a method for lysing cells, and a kit for the isolation and purification of nucleic acids.
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