| abstract |
The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single stranded mRNA encoding an iPS cell inducer. A purified RNA preparation will preferably i) activate an immune response in somatic cells, ii) reduce the expression of single stranded mRNA in somatic cells, and / or iii) somatic cells. There is virtually no RNA contaminant molecule that activates the RNA sensor. In certain embodiments, the purified RNA preparation is substantially free of partial mRNA, double stranded RNA, uncapped RNA molecules, and / or single stranded running mRNA. |