| abstract |
A method for modifying and improving a cell biosynthesis mechanism more efficiently and effectively is provided. The composition comprises a composition and method for producing components of a protein biosynthetic mechanism comprising an orthogonal tRNA, an orthogonal aminoacyl tRNA synthetase, and an orthogonal tRNA / synthetase pair. The composition comprises a novel orthogonal tRNA-aminoacyl tRNA synthetase pair. New orthogonal pairs can be used to incorporate unnatural amino acids into polypeptides in vivo. [Selection figure] None |