| abstract |
[PROBLEMS] To be unique in human ES / iPS cells that enables 1) efficient differentiation induction method by gene transfer, 2) high-purity isolation of target cells, 3) overcoming tumor formation (undifferentiated / cancer source cells). Provides the developed adenoviral vector (ADV) technology. A method for inducing cardiomyocytes from human ES cells or human iPS cells, RSV promoter or CMV, wherein Mesp1 is introduced in the step of forming embryoid bodies of human ES cells or human iPS cells. Introducing into the human pluripotent stem cell an expression cassette in which a marker gene is linked downstream of the promoter; culturing the human pluripotent stem cell; And a method for identifying differentiated cells in a human pluripotent stem cell culture, comprising: determining cells expressing the marker gene as differentiated cells. [Selection figure] None |