abstract |
The present invention provides a hepatitis C virus gene, a replicon RNA derived from the gene, a replicon-replicating cell into which the replicon RNA has been introduced, and a drug screening method using the replicon-replicating cell. A leucine of the 1804th in the amino acid sequence of a polynucleotide consisting of a specific base sequence, a replicon RNA containing a polynucleotide encoding a polypeptide consisting of a specific amino acid sequence, or a polyprotein of hepatitis C virus, and A replicon-replicating cell is produced by introducing a replicon RNA of genotype 1b containing a nucleotide encoding 1966 lysine and a polynucleotide encoding NS4B protein into the cell, and drug screening is performed by the replicon-replicating cell. I do. [Selection figure] None |