| abstract |
A method for increasing the efficiency of the production of secondary alcohols, ketones or optically active secondary alcohols. In the amino acid sequence of a secondary alcohol dehydrogenase derived from Candida parapsilos, for example, the amino acid at position 41, position 49, position 77, position 90, position 97 and / or position 113 is replaced with another amino acid. By substituting for, a secondary alcohol dehydrogenase mutant protein (mutant enzyme) that retains the secondary alcohol dehydrogenase and has organic solvent resistance is obtained. This enzyme is useful for the production of optically active alcohols using ketone as a raw material. [Selection figure] None |