abstract |
PROBLEM TO BE SOLVED: To provide methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery, and viral infection Providing assessment of screening for drugs that affect sex and / or replication. At the beginning of vector production, the AAV producer cell contains one or more AAV packaging genes, an AAV vector containing a heterologous (ie, non-AAV) transgene of interest, and a helper virus such as an adenovirus. . The AAV vector preparation produced is generally not replication competent, but can mediate delivery of the transgene of interest (eg, a therapeutic gene) to any wide range of tissues and cells. AAV vector preparations are substantially free of helper virus and helper virus proteins and cellular proteins and other contaminants. [Selection figure] None |