| abstract |
Provided is a hydrolase having excellent optical selectivity, which is used in an organic synthesis reaction for producing an optically active α-substituted-β-amino acid derivative and the like. The following enzyme (a) or (b): (A) An enzyme having an amino acid sequence represented by at least 1st to 362th in a specific amino acid sequence, wherein the 277th tyrosine is substituted with alanine, tryptophan, isoleucine, or histidine. (B) a specific amino acid sequence having at least the first to 362th amino acid sequences, wherein the 277th tyrosine is substituted with an amino acid different from tyrosine and has the ability to hydrolyze a substrate; Characteristic enzyme. [Selection figure] None |