http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013066390-A

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34
filingDate 2011-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15f0b0a9c5d4b93fd8dfaa30422132ec
publicationDate 2013-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2013066390-A
titleOfInvention Method for measuring the concentrations of hippuric acid and methylhippuric acid in biological samples
abstract A method for measuring the respective concentrations of hippuric acid and methylhippuric acid in a biological sample is provided. (1) An enzyme that hydrolyzes hippuric acid into benzoic acid and glycine and hydrolyzes methylhippuric acid into methylbenzoic acid (toluic acid) and glycine is allowed to act on a biological sample collected from a living body. (2) The reaction solution obtained in the step (1) is oxidized, (3) the reaction solution obtained in the step (2), D-amino acid and D-amino acid oxidase are mixed, and (4) (3) The concentration of hydrogen peroxide generated in the process is specified. In addition to the steps (5) (2) to (4), the reaction solution obtained in the step (1), D-amino acid and D-amino acid oxidase are mixed, and (6) in the step (5). The concentration of the generated hydrogen peroxide solution is specified, and the concentration of the hydrogen peroxide solution obtained in the steps (7) and (4) and the concentration of the hydrogen peroxide solution obtained in the step (6) The respective concentrations of hippuric acid and methyl hippuric acid are calculated. [Selection] Figure 1
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