| abstract |
The present invention provides a method that does not require the addition of a surfactant as a separate component in a production method for a combination vaccine containing a nonionic surfactant. In preparing HBsAg for use in combination vaccines, it is known to add a nonionic surfactant after purification of HBsAg. However, it is not optimal to add a surfactant after purification of HBsAg. This is because it requires another processing step during manufacture. Therefore, the present invention uses a surfactant during the purification of HBsAg. [Selection] Figure 3 |