http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013042722-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a2583bd553a6ef8f66dd0718f5dac4be |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
| filingDate | 2011-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43e5de755e4bc4cb557fe3be3335ed6a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_796fd58bbb4989710ee09946fb6c6b69 |
| publicationDate | 2013-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2013042722-A |
| titleOfInvention | Amino acid bioassay based on protein expression |
| abstract | [PROBLEMS] To provide an amino acid quantification method that enables high-sensitivity and high-precision measurement in a short time using lactic acid bacteria having auxotrophy related to amino acids, and that is less susceptible to factors that affect the growth of bacteria. To do. A method for quantifying amino acids in a specimen, comprising the following steps. Introducing into the lactic acid bacterium a protein gene whose expression level can be quantified in a state in which the expression can be regulated to obtain a recombinant lactic acid bacterium, step (A), expression of the protein gene in a medium containing the amino acid to be measured at a predetermined concentration Step (B) for obtaining a correlation between the amount of protein produced by the recombinant lactic acid bacteria and the amino acid concentration under induction conditions, culturing the recombinant lactic acid bacteria in a medium not containing the measurement target amino acid, Included in the sample from the amount of protein measured in the step (C) based on the correlation obtained in the step (C) and the step (B) of measuring the amount of protein contained in the culture solution after culturing Determining the amount of amino acid to be obtained (D). [Selection figure] None |
| priorityDate | 2011-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 162.