| abstract |
The present invention provides improved means for producing recombinant BMP and other TGF-β family proteins in vitro using prokaryotic and eukaryotic hosts. A component of a latent TGF-β family member fusion protein for refolding under appropriate refolding conditions, including an N-terminal truncated TGF-β family protein, the shortened TGF-β The family protein comprises a TGF-β family protein C-terminal 7 cysteine domain comprising a finger 1 subdomain, a finger 2 subdomain, and a heel subdomain; and a cleavable modified leader operably linked to the C-terminal domain A leader sequence that inhibits a biological activity associated with the C-terminal domain, wherein the C-terminal domain is activated upon cleavage of some or all of the leader sequence A fusion protein comprising [Selection figure] None |