abstract |
A method for detecting and isolating AAV sequences in samples of DNA obtained from tissues or cells, and AAV sequences identified by the method and vectors constructed using these methods are provided. A method that utilizes the ability of adeno-associated virus (AAV) to enter the nucleus and integrate into cellular DNA in the absence of co-infection of helper virus and establish latent infection. A polymerase chain reaction (PCR) based strategy for detection, identification and / or isolation of AAV sequences from DNA from human and non-human primate origin tissues and other sources. Advantageously, detection, identification and / or isolation of other integrated viral and non-viral sequences. [Selection figure] None |