| abstract |
A light-induced dielectrophoresis (DEP) apparatus and separation method by light pattern driving is described, wherein the apparatus is a particle or cell apparatus or a selection based on traits contrasted with a DEP reaction. Embodiments of the device use a DEP electric field pattern in combination with a micro-flow laminar flow, and the reaction of particles from a heterogeneous mixture according to the relative reaction of the particles to one or more DEP electric fields without damage to living cells. Perform measurement, separation, isolation, and extraction. Suitable OET-DEP devices generally have a planar liquid-filled structure having one or more photoconductive portions, with a gradient of the DEP electric field selected according to the input and the plurality of output fluid channels. Along with this, incident light is converted into a localized virtual electrode. This light pattern is dynamically generated and provides a number of manipulating structures that can manipulate single particles and cells or groups of particles / cells. This method is particularly useful for the selection and extraction of optimal sperm and embryo candidates based on their suitability for use with existing artificial regeneration processes, and for the exclusion of defective or non-viable gametes. Suitable. |