http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2012517813-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2010-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2012-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2012517813-A |
| titleOfInvention | Template-independent ligation of single-stranded DNA |
| abstract | The present invention provides ligation reaction mixtures, methods, and kits for improved template-independent intramolecular ligation (circularization) of linear ssDNA, wherein the linear ssDNA is a denatured gDNA fragment or heat. First strand cDNA generated by reverse transcription of RNA using denatured RNA ligase or the like is included. Circular ssDNA molecules obtained using improved ligation reaction mixtures and methods can be used as templates for amplification by inverse PCR, rolling circle replication, transcription, or massively parallel DNA sequencing, and the like. The present invention includes, for example, gene expression analysis by qPCR or analysis of gDNA copy number change using a microarray, and research, screening, medical diagnosis, theranostics, individual medical treatment or bleeding, human or animal medicine, forensic medicine Or for the detection or quantification of a specific nucleic acid sequence for purposes such as agriculture. [Selection figure] None |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016525360-A |
| priorityDate | 2009-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 744.