| abstract |
The present disclosure provides a modified antibody comprising an antibody or antibody fragment (AB) modified with a masking moiety (MM). Such modified antibodies can be further coupled with a cleavable moiety (CM) to yield an activatable antibody (AA), which is cleaved, reduced or photolyzed, or otherwise modified. be able to. AA is such that AB becomes more accessible to the target after removal of MM, for example by cleavage, reduction or photolysis of CM in the presence of an agent capable of cleaving, reducing or photolyzing CM. Can exhibit an activatable conformation. The disclosure further provides methods of making and using such modified and activatable antibodies. |