| abstract |
The present invention relates to a method for generating an immunoglobulin sequence for a cell-associated antigen, more specifically an antigen immobilized on a membrane. The invention also provides immunoglobulin sequences obtainable by the methods of the invention. Specifically, the present invention relates to the generation of immunoglobulin sequences by use of DNA vaccination. More particularly, the present invention relates to the generation in camelids of immunoglobulin sequences directed by DNA vaccination, preferably directed to cell-associated antigens, particularly antigens with multiple transmembrane domains including GPCRs and ion channels. Furthermore, the present invention relates to cell-associated antigens, more specifically antigens immobilized on membranes such as GPCRs and ion channels, more preferably the above immunoglobulin sequences for ion channels. [Selection figure] None |