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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
filingDate 2009-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2012-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2012512406-A
titleOfInvention In-process control in EPO manufacturing method
abstract The present invention includes the following steps: a) A step of subjecting a sample containing erythropoietin to isoelectric focusing in a gel over a pH range having a lower limit of 2.5 to 3.5 and an upper limit of 5 to 8. In this case, the sample containing erythropoietin is derived from the culture supernatant of eukaryotic cells producing erythropoietin; b) transferring the protein contained in the gel and separated to the membrane; c) In-process the culture supernatant of eukaryotic cells producing erythropoietin within the scope of the method of determining the isoform composition of erythropoietin, including the step of detecting the erythropoietin bound to the membrane with a specific antibody It relates to a method of controlling.
priorityDate 2008-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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