| abstract |
The present disclosure relates to immunoglobulins and compositions having reduced aggregation, methods for generating such immunoglobulins using computer tools, and methods for using such immunoglobulins, particularly in the treatment and prevention of disease. About. In one aspect, the invention has (i) a spatial aggregation tendency (5 radius radius sphere) of at least 0.15 or (ii) a spatial aggregation tendency (5 radius radius sphere) greater than 0.0. A reduction comprising at least one aggregation-reducing mutation in a residue in the conserved domain of an immunoglobulin having and having a spatial aggregation tendency of at least 0.15 (5 radius radius sphere) within 5 km of the residue Modified and / or isolated immunoglobulins having a tendency to aggregate are provided. |