| abstract |
The present invention relates to a method for identifying a compound that inhibits activation of RAC GTPase by DOCK5 protein, comprising the step of (i) co-expressing DOCK5 and RAC protein in a cell (wherein the DOCK5 protein is not expressed). Induces conversion of active RAC (this inactive RAC is bound to GDP) to active RAC (this active RAC is bound to GTP)), (ii) contacting the cell with the compound Or (iii) determining the conversion of inactive RAC to active RAC in the presence or absence of the compound, and (iv) a compound that inhibits conversion of inactive RAC to active RAC To a method comprising the step of selecting. The compounds are useful for the treatment of bone defect related diseases. |