abstract |
A method for immunologically measuring HbA1c that is applicable to an immunochromatography method, which is a simple measurement system, is provided. An immunochromatographic measurement method for HbA1c comprising the following steps (A), (B), (C) and (D). (A) A measurement sample containing red blood cells is treated with a surfactant having the property of exposing the N-terminus of hemoglobin β chain to the protein surface. (B) The measurement sample obtained in step (A) is water-insoluble. The step (C) of contacting with the cyclic polysaccharide in the state of (C) The measurement sample obtained in the step (B) is an antibody that recognizes the N-terminus of hemoglobin labeled with particles or other than the N-terminus of hemoglobin labeled with particles Step (D) for contacting with any antibody that recognizes the antibody that recognizes the N-terminus of hemoglobin labeled with particles and / or other than the N-terminus of hemoglobin labeled with particles formed in step (C) For detecting immune complex between antibody and HbA1c [selection figure] None |