abstract |
Screening assays, compounds, and methods for altering gene expression and protein levels are provided. A nucleic acid sequence encoding a high level mammalian expression vector, an intron, and a reporter polypeptide, wherein the nucleic acid sequence encoding a reporter polypeptide is proximally linked to a target untranslated region (UTR). Nucleic acid constructs. Assays to screen for agents that can modulate gene expression in a UTR-dependent manner, and agents that can modulate gene expression. [Selection figure] None |