| abstract |
When using reporter fluorophores in reporter / quencher pairs in hybridization applications, undesirable properties, difficult-to-separate mixtures, positive charge, difficulty in synthesis, instability during oligonucleotide synthesis, or Provided are reagents for oligonucleotide probes that solve problems such as overlap of emission wavelength with other desirable reporters. Oligonucleotide-fluorophore wherein the fluorophore moiety has an emission wavelength in the range of about (300) to about (800) nm and / or the quencher has a substituted 4- (phenyldiazenyl) phenylamine structure -Quencher conjugate. [Selection figure] None |