| abstract |
A method for identifying targets for the development of therapeutic drugs to address disease states. Methods for identifying potential target host genes and encoded proteins for therapeutic intervention use gene search vectors that are either lentiviral or MMLV-based, and Used to collate the entire cell genome without prior knowledge. This random homozygous gene perturbation (RUGP) technique is used to identify potential host targets for intervention on influenza, HIV, and other viral infections. Thermal asymmetric interlace (TAIL) PCR is used to reduce the period for identification of a promising target from months to weeks or less. Target specific substances (including PTCH1, Robo1, and Nedd4). [Selection figure] None |