| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_28ab2241f8ec56c30a295e61ff821c43 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483 |
| filingDate |
2010-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f4e0cee8cf6d61eaca8c108d5992c86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_baf1a2a38245756b2a18c57ea57523d6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8dfa93bf1e3af3c9578cd9d4a47862a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d7af7647fab0d80efee874ea06acd28 |
| publicationDate |
2012-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2012127694-A |
| titleOfInvention |
Method for detecting intracellular ubiquitination |
| abstract |
A method for quantitatively detecting polyubiquitination and / or deubiquitination of a target protein in a cell is provided. A method for detecting intracellular polyubiquitination and / or deubiquitination of a target protein, comprising the following steps: 1) Fusion of a target protein (A) and a tag (T 1 ) A transformed cell that expresses both a protein (X) and a fusion protein (Y) of ubiquitin (B) and a tag (T 2 ), wherein the fusion protein (X) is labeled with a fluorescent dye (D 1 ); In addition, a transformed cell labeled with the fluorescent dye (D 2 ) is prepared for the fusion protein (Y), and 2 ) the transformation is performed under conditions where polyubiquitination and / or deubiquitination of the target protein is performed. Incubate the cells, 3) irradiate the transformed cells with laser light, and 4) measure the fluorescence lifetime of the fluorescence emitted by the transformed cells. [Selection figure] None |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110835641-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108536084-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014025764-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110835641-A |
| priorityDate |
2010-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |