http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2012125200-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7b097bfd3f350ed27ffa53e1f114d1a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fcb393875d4c6ff4098a102cc1304b44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d89d0f1dc7070794dd8f76afe7100d14 |
| publicationDate | 2012-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2012125200-A |
| titleOfInvention | Analysis method of target nucleic acid |
| abstract | [Problem] To provide a method for nucleic acid analysis using a single standard sample that can be easily manufactured and stored for a long period of time, including a series of steps of nucleic acid extraction / purification, nucleic acid amplification reaction, and amplification product detection. . (A) a step of extracting a nucleic acid from a cell sample to be analyzed; and (b) a nucleic acid amplification reaction using the nucleic acid extracted in step (a) or a nucleic acid synthesized using the nucleic acid as a template as a template. A step of amplifying a target nucleic acid and detecting an amplification product, and (c) an expression vector into which a standard nucleic acid is incorporated is introduced, and fluctuations in the expression profile are suppressed while retaining the nucleic acid inside the cell. A step of extracting nucleic acid from a standard cell sample comprising transformed cells immersed in an inactivating liquid, and (d) a nucleic acid extracted in step (c) or a nucleic acid synthesized using the nucleic acid as a template. A method for analyzing a target nucleic acid, comprising a step of amplifying a standard nucleic acid by a nucleic acid amplification reaction using a template and detecting the obtained amplification product. [Selection figure] None |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015060511-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10072286-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019004814-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140118680-A |
| priorityDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 464.