| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_430c89d97f6839f5364904e710af9704 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2011-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4d703d2e5ef09e169f754622826f9bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_badbed9b98669d5447876b4576252a99
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24bbc9521ca202e6582b332a2a5a1090
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90714610928063a795a9b72545813f0a |
| publicationDate |
2012-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2012040029-A |
| titleOfInvention |
Mutation detection method and kit used therefor |
| abstract |
PROBLEM TO BE SOLVED: To provide a method for detecting a mutation having excellent detection sensitivity using Tm analysis. A sample containing a detection target DNA in which a detection site is mutated and a non-detection target DNA in which the detection site is unmutated is complementary to a detection target sequence containing the mutated detection site. A detection probe composed of a polynucleotide and a non-detection target polynucleotide containing an unmutated detection site containing the detection site are added, and the detection probe is hybridized to the DNA. Then, the presence or absence of mutation is determined by heating the hybrid of the DNA and the detection probe to measure signal fluctuations accompanying temperature rise, and analyzing the signal fluctuations to determine the Tm value. To do. [Selection] Figure 1 |
| priorityDate |
2006-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |