| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7dffbd04747d5d9b9492e7f2ac61db6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e8d2afa63dbd8bbb90545722d3f54c1 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-235
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-05
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0393 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-873
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5073
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-873
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate |
2011-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b01f036ad426c617b9f4577f828d7a24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_972e3812c19932d3f7ecba86533c524b |
| publicationDate |
2012-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2012024088-A |
| titleOfInvention |
Rat embryonic stem cells |
| abstract |
A rat embryonic stem cell, a method for establishing the rat embryonic stem cell, and the like are provided. A process including the following steps (A) to (D) is performed under culture conditions using a culture solution having a serum concentration of 2% or less, and rLIF is contained in steps (B) to (D): A rat embryonic stem cell obtained by a production method characterized by using a culture solution to: (A) An inner cell mass formed by culturing a rat blastocyst using a culture solution not containing rLIF, The process of dissociating while holding the agglomerates. (B) A step of culturing primary embryonic stem cells that have emerged by culturing the dissociated inner cell mass until they can be passaged. (C) A step of dissociating, subculturing and culturing primary embryonic stem cells that can be passaged in a state in which cell clumps are retained. (D) A step of establishing embryonic stem cells by further subculturing and culturing. [Selection figure] None |
| priorityDate |
2004-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |