http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011530279-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-025 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
filingDate | 2009-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2011-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2011530279-A |
titleOfInvention | Determining the sensitivity of cells to drugs |
abstract | The present invention comprises (i) treating a patient sample or cell line or a part thereof with a deoxyribonucleoside kinase-dependent drug; (ii) lysing a patient sample or cell line cell derived from step (i); (Iii) optionally mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacterium incorporating a gene encoding deoxyribonucleoside kinase; (iv) cell lysis from (ii) Mixing a part of the product with a bioluminescent reporter bacterium incorporating a gene encoding deoxyribonucleoside kinase, and a deoxyribonucleoside kinase transcription promoter; (v) a part of the cell lysate from step (ii), and deoxyribonucleoside Bioluminescent report incorporating a gene encoding a kinase A step of mixing a bacterium, a deoxyribonucleoside kinase transcription promoter and a dephosphorylating agent; and (vi) measuring the bioluminescence of each of the mixtures derived from steps (iii) to (v), wherein A comparative level of bioluminescence for each of the mixtures provides a measure of resistance or sensitivity to the drug.) Provides an in vitro method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug To do. |
priorityDate | 2008-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 254.