http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011527567-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate | 2009-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2011527567-A |
| titleOfInvention | Recombinant expression vector for animal cells |
| abstract | The present invention relates to a recombinant expression vector for animal cells comprising a DHFR-encoding nucleotide sequence operably linked to a dihydrofolate reductase (DHFR) promoter, an animal cell strain transformed with the vector, and The present invention relates to a method for producing a target protein. The vector of the present invention makes it possible to effectively select a cell line clone in which a foreign gene is amplified together with a DHFR gene even under a significantly lower methotrexate concentration than existing animal cell expression vectors. In addition, according to the present invention, in the process of establishing a protein-producing cell line, the use of a lower concentration of methotrexate can secure a highly productive cell line in a short period of time. Show. [Selection] Figure 3a |
| priorityDate | 2008-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 880.