http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011514169-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0062 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0647 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0789 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 |
| filingDate | 2009-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2011514169-A |
| titleOfInvention | Microcarrier for stem cell culture |
| abstract | We disclose a particle comprising a matrix coated thereon and having a positive charge, the size of which allows for the assembly of primate or human stem cells attached thereto. The particles can include substantially elongated, cylindrical or rod-shaped particles having a longest dimension of 50 μm to 400 μm, for example about 200 μm. It can have a cross-sectional dimension of 20 μm to 30 μm. The particles can include substantially compact or spherical shaped particles having a size of about 20 μm to about 120 μm, for example about 65 μm. The present inventors provide a method for propagating primate or human stem cells, comprising preparing first and second primate or human stem cells attached to respective first and second particles, Contacting a primate or human stem cell with a second primate or human stem cell to form a cell aggregate, and culturing the aggregate to propagate at least one passage of the primate or human stem cell. A method of including is also disclosed. Also disclosed is a method of propagating human embryonic stem cells (hESC) in long-term suspension culture using microcarriers coated in Matrigel or hyaluronic acid. We also disclose a method for differentiating stem cells. [Selection] Figure 1 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020162551-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7100853-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020203753-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017500013-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2018225703-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018225703-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7284985-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021510527-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016526387-A |
| priorityDate | 2008-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 684.