http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011512515-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-036 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-07 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5076 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2009-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2011512515-A |
| titleOfInvention | Method for identifying secreted granules of different ages |
| abstract | The present invention relates to an in vitro method for detecting different age SGs in a cell by distinguishing and labeling the SGs according to the age of the secretory granules (SG) of different age. And the following steps: (A) A SG-specific (poly) peptide that has a binding site common to at least three different substances A, B and C that form SG and are covalently bound to it: 2. (i) comprising a (poly) peptide specific for SG, and (ii) a (poly) peptide having a binding site common to at least three different substances A, substance B and substance C covalently bound thereto. A cell capable of expressing a fusion protein; wherein said substance is capable of penetrating the cell membrane, and at least substance A and substance C are detectable by separate means, targeting a binding site Contacting with substance A; (B) a step of saturating the binding site by contacting the cell with a substance B that blocks a binding site that is not bound by the substance A; (C) allowing unbound substance B to be removed from the cell, or removing unbound substance B from the cell; (D) contacting the cell with a substance C that targets a binding site on a (poly) peptide or fusion protein and making it available for binding after step (b); and (e) detectable Detecting for the presence of both substance A and substance C; [where simultaneous detection of both substance A and substance C indicates the presence of two populations of SG with different ages] Relating to said in vitro method. Furthermore, the present invention relates to an in vitro method for studying the effects of stimuli in cells on SG that are differentially labeled according to the age of the secretory granules (SG). [Selection figure] None |
| priorityDate | 2008-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1067.