| abstract |
An integration-deficient retroviral vector transfer cassette lacking a functional polyprint lact (PPT) is provided. Also, an isolated nucleic acid consisting of a heterologous nucleotide sequence, one or two retroviral long terminal repeats (LTR), a packaging signal, a rev response element and a eukaryotic promoter; A recombinant retroviral particle and their mRNA; a retroviral vector kit; and an integration-deficient vector particle, gene expression of a predetermined nucleotide sequence, and the predetermined nucleotide sequence in the host cell genome Methods are provided for insertion in a site-specific or non-specific manner. [Selection figure] None |