| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69ece09bafee7e0c49d1f7951a3faa2c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-475 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate |
2011-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b03e739bbe7193b18a0acec115ef81bf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1996dff1348e504e6e89a486819d531
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_228b8808854953cb7d15c158aa4a8159
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3187d054a13f203297773c05290cf73
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2df079420f47c6f14061cd433b21de5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c661364a499b3f805b91b0b8cc7db1b4 |
| publicationDate |
2011-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2011254831-A |
| titleOfInvention |
Recombinant production of heparin binding protein |
| abstract |
A method is provided for recovering and purifying refolded heparin-binding protein produced in heterologous host cells. A heparin-binding protein is isolated from the periplasm of a prokaryotic cell culture, solubilized in a first buffer solution containing a chaotropic agent and a reducing agent, and then a sulfated polyanion such as a chaotropic agent and dextran sulfate is used. A method for recovering the refolded protein by incubating in a second buffer solution containing ions. [Selection figure] None |
| priorityDate |
2005-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |