| abstract |
A powdered nutrient medium, a medium additive, and a medium subgroup formulation are provided. Contains cell culture fluid additives (including powdered serum such as powdered fetal bovine serum (FBS)), media subgroup formulations, and all essential trophic factors including lipid components that facilitate in vitro culture of cells Cell culture solution. Prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells, and animal cells (including human cells) are cultured using dry powder nutrient media, media additives, media subgroups, and buffer formulations. Prior to that, aseptic powder medium, medium additives (especially powdered serum such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powder growth factors (EGF, FGF, etc.) ), Etc.), media subgroups, and buffer formulations are produced, and sterilization is achieved by gamma irradiation, and media, additives, media subgroups, and buffers are powdered. [Selection figure] None |