http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011229488-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f97f0fa258a008bf056ad04b7f380dda |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ff3397493066331744c70ce2bd3d006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0cf2af66921cd7008274621cca52f8b0 |
| publicationDate | 2011-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2011229488-A |
| titleOfInvention | Real-time nucleic acid amplification by droplet manipulation |
| abstract | The present invention provides a real-time nucleic acid amplification method capable of detecting fluorescence obtained in a nucleic acid amplification method carried out in droplets in a completely sealed system at high speed and with high accuracy. A nucleic acid amplification reaction method for performing a nucleic acid amplification reaction in a droplet existing in a container, wherein the droplet comprises a nucleic acid amplification reaction solution containing nucleic acid to be amplified and magnetic particles, and the container A droplet encapsulating medium that does not mix with the nucleic acid amplification reaction liquid that forms the droplet is held and has a transport surface provided with a temperature gradient. At the start of the nucleic acid amplification reaction, the droplet and the droplet are enclosed. At least the droplet encapsulating medium of the medium contains a fluorescent dye, and at the start and maintenance of the nucleic acid amplification reaction, a magnetic field is generated by the magnetic field applying means to convey the droplet together with the magnetic particles and to synthesize nucleic acid on the conveying surface. A real-time nucleic acid amplification reaction method in which the temperature of a reaction solution is controlled by placing droplets at a temperature point at which reaction is initiated and maintained. [Selection] Figure 3 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9623410-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017051939-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110872551-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013217699-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10369563-B2 |
| priorityDate | 2010-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 97.