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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06T1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06T7-60
filingDate 2010-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbc1500406d61fbacf94d3f4da02bac0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c810f5204dcd52f2ae399a33b957e3c
publicationDate 2011-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2011186750-A
titleOfInvention Method and apparatus for quantitative analysis of molecules to be measured
abstract A method for quantitatively analyzing a molecule to be measured in a cell, which is versatile and has a high throughput, and a program, an apparatus, and a system for executing the method are developed. According to the method of extracting a cell outline of the present invention, for each cell membrane stained image pixel, a standard deviation of cell membrane staining brightness of pixels in the adjacent region is calculated, and the standard deviation of the pixel deviation is calculated. Whether the value corresponding to the peak of the curve fitted to the frequency distribution in the vicinity of the mode value is an estimated value of the standard deviation of the cell membrane staining luminance of the background region, the arithmetic average value of the cell membrane staining luminance of the background preliminary candidate pixels And determining the sum of the offset determined based on the estimated value as a cell pixel threshold, and determining a cell membrane stained image pixel having cell membrane staining brightness equal to or higher than the cell pixel threshold as a cell candidate pixel. [Selection figure] None
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