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publicationDate 2011-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2011185842-A
titleOfInvention Low oxygen region analysis method and apparatus for biological samples by time-resolved measurement of photoinduced autofluorescence
abstract An object of the present invention is to enable measurement of an oxygen concentration of a substance to be measured with high accuracy without being affected by the intensity of excitation light, the concentration of a fluorescent substance, and the surrounding environment. Pulse excitation light L1 including a wavelength capable of exciting a fluorescent material having a fluorescence lifetime Tf of 4.8 nanoseconds or longer contained in a biological material is generated, and the pulse excitation light L1 is generated at a predetermined position of the biological material. The light L3 containing the fluorescence Lf generated from the fluorescent material excited by the irradiation is received, the intensity of the fluorescence Lf is time-resolved to calculate the fluorescence lifetime Tf of the fluorescence Lf, and the biological lifetime of the biological material is calculated from the fluorescence lifetime Tf. Measure the oxygen concentration. [Selection] Figure 1
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