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publicationDate 2011-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2011160802-A
titleOfInvention Method for controlling pH, osmolarity and dissolved carbon dioxide levels in a mammalian cell culture process to increase cell viability and biological product yield
abstract A method is provided for controlling dissolved carbon dioxide levels in mammalian cell culture processes, limiting osmolarity, increasing cell growth, viability and density, and increasing biological product concentration and yield. To do. Such control of dissolved carbon dioxide levels and pH in mammalian cell culture processes, and the ability to limit the resulting osmolarity, is an alternative pH control technique and CO 2 separation technology throughout mammalian cell culture processes. Is achieved by adopting The pH control technique and carbon dioxide separation are performed without generating bubbles and with little or no damage to mammalian cells. [Selection] Figure 1
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