| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e44333393916a1ef9c9f10566af4833d |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-02 |
| filingDate |
2011-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4142e76bbd06faa7ff711425fa90d77a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4b7c625f362251dc3ed9df28d59c9c5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bce90d61c723e7b46d31444bf6ac178 |
| publicationDate |
2011-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2011139714-A |
| titleOfInvention |
Cell lines for expressing enzymes useful for the preparation of amidation products |
| abstract |
A cell line for expressing peptidylglycine α-amidated monooxygenase (PAM), or one of its two catalytic domains. High levels of enzyme expression are achieved using low protein tissue culture media from non-animal sources. Robust two-step downstream purification results in high enzyme purity. The resulting PAM or its PHM catalytic domain is X-α-hydroxy-Gly or X-NH 2, where X is a peptide or any chemical compound having a carbonyl group to which a glycine group can be covalently bonded. -Used to catalyze enzymatic conversion of Gly conversion. A preferred cell line preparation method is also shown. [Selection] Figure 1 |
| priorityDate |
2005-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |