http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011050401-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad2cd796f8c4dcb425ddcc68707d3444 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_148963eee3a298589a747bf33f813d23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4620a8b316dfecd358ca173168600edf |
| publicationDate | 2011-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2011050401-A |
| titleOfInvention | Rapid detection method of nucleic acid |
| abstract | In a conventional method using a nucleic acid probe or a nucleic acid primer whose fluorescence is quenched upon hybridization with a target nucleic acid, the nucleic acid probe inhibits the nucleic acid amplification process, particularly the extension process, so that sensitivity is reduced when shortening the time. There is a problem that the target nucleic acid-specific detection is hindered by non-specific amplification of the nucleic acid primer. An object of the present invention is to overcome these problems and to rapidly detect a target nucleic acid while maintaining specificity and high sensitivity. Specificity and high sensitivity are maintained by adjusting and optimizing the relationship between the Tm value of the first primer and the second primer and the Tm value of the labeled probe, and the relationship between the primer concentration and the labeled probe concentration. While detecting the target nucleic acid rapidly. [Selection figure] None |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011083286-A |
| priorityDate | 2007-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.