| abstract |
Methods of screening for inhibitors of endoplasmic reticulum (ER) stress are provided. These methods include adding thapsigargin, which induces ER stress, and a test agent to mammalian cells in a multiwell plate. Cell viability can be easily monitored by measuring intracellular ATP content using a bioluminescent reagent. Screening a commercially available 50,000 compound library has led to the identification of 93 hit compounds that have been subjected to secondary assays and their ability to protect cells from thapsigargin-induced cell death. confirmed. |