| abstract |
The present invention relates to an ex vivo method for making a bispecific antibody comprising the steps of: a) providing a first antibody having a first binding specificity, comprising: An antibody comprising an IgG4-like CH3 region, b) providing a second antibody having a second binding specificity that is different from the first binding specificity, wherein the second antibody is an IgG4-like Including a CH3 region, c) incubating the first antibody and the second antibody together under reducing conditions that allow cysteines in the core hinge region to undergo isomerization of disulfide bonds, and d ) Obtaining bispecific antibodies. The present invention further relates to a bispecific antibody obtainable by the method of the present invention. |