| abstract |
In one aspect, the present invention provides methods and compositions for expressing small RNA molecules in cells using retroviral vectors (FIG. IA). The methods of the invention can be used to express small interfering RNA (siRNA) in cells. In a further aspect, the present invention provides a method of producing a lRNA encoding a lentivirus, wherein the siRNA activity can interfere with the lentivirus life cycle. In yet a further aspect, the invention provides a method for expressing a small RNA molecule, such as siRNA, that can be regulated to reduce CCR5 in a cell, wherein the expression of the small RNA molecule is relatively Methods are provided that are not cytotoxic. The invention also includes small RNA molecules, such as siRNA, that can be regulated to reduce CCR5 that are relatively non-cytotoxic to cells. |