| abstract |
Methods and kits for nucleic acid analysis are provided. In an exemplary method, a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer includes a probe element specific for a portion of the target nucleic acid and a template specific primer region. This probe element is located 5 'of the template-specific primer region, thus allowing the probe element to hybridize with the hairpin-forming moiety and measuring fluorescence from the dsDNA binding dye as the mixture is heated To generate a melting curve for the probe element, where the dye is not covalently bound to the first primer and the shape of the melting curve is analyzed. The kit may include one or more first and second primers, a dsDNA binding dye, a polymerase, and dNTPs. |