http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010517514-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-501 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate | 2008-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2010-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2010517514-A |
| titleOfInvention | Preparation method of nucleic acid molecule |
| abstract | The present invention generally relates to a method of making a single-stranded nucleic acid molecule after enhanced solid phase polynucleotide amplification. The present invention uses amplification reactions using primers that have differential priming characteristics under specific annealing conditions, or an immobilized primer nested between two aqueous phase primers. Therefore, the primer design enhances the involvement of the solid support primer compared to the aqueous phase primer. Furthermore, the present invention provides a method for labeling a solid matrix with single and double stranded nucleic acid molecules. Kits for producing single-stranded nucleic acid molecules and performing amplification reactions also form part of the present invention. Furthermore, the present invention provides an amplification system for making a single stranded nucleic acid molecule that may be labeled with a reporter molecule, and the use of this single stranded nucleic acid molecule, particularly as a label, primer, and probe. To do. |
| priorityDate | 2007-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.