abstract |
The present invention describes oligonucleotides that bind to microRNA target sites in a target RNA (eg, mRNA). The oligonucleotides of the invention can mediate degradation of the target RNA by RNase H, or mediate RNAi of the target RNA, or block the regulation of the target RNA by microRNA. The oligonucleotides of the present invention are useful, for example, as research tools for investigating microRNA: mRNA interactions and developing therapeutics. The present invention also relates to a method for identifying a microRNA target site, a method for confirming a microRNA target site, a method for identifying an oligonucleotide of the present invention, and a method for modulating the activity of a target RNA using the oligonucleotide of the present invention. It is described. |